SFO-1 and DHA-1 genes Detection in β-lactamases-producing Klebsiella pneumoniae Causing Blood Stream Infections Acquired in Intensive Care Units in Mansoura University Hospitals
Document Type : New and original researches in the field of Microbiology.
Department of Medical Microbiology & Immunology, Faculty of Medicine, Mansoura University, Egypt
Abstract
Objectives: The study aimed to detect SFO-1 and DHA-1 genes and to differentiate AmpC with overlapped ESBL isolates. Also, it identified the risk factors aiding in emergence the ESBL producing K.pneumoniae causing blood stream infections in ICUs of Mansoura University Hospitals (MUHs). Methodology: This is a prospective study that enrolled 520 blood samples. Double disk synergy test (DDST) and Modified three-dimensional test were performed. Genotypic detection of SFO-1, Class A ESBL, and DHA-1, AmpC β-lactamase was done. Results: 520 septicemic patients were enrolled with significant correlation for adults’ acquisition of infection. The main bacteria isolates causing nosocomial infection in septicemic patients admitted to ICU were S. aureus, E. coli and K. pneumoniae. A significant difference in distribution of ESBL and AmpC β-lactamases was detected with significant association between impenem resistance and prevalence of ESBL in those patients. There was a low-occurrence of ESBL SFO-1 and DHA-1 detected in clinical samples. Surgical intervention and CVC were the significant risk factors for presence of ESBL but previous antibiotics and hospital stay were non-significant effectors for presence of ESBL. Conclusion: Though SFO-1 is a low-prevalence ESBL, it has been taken by a plasmid with many other multiple resistance determinants including many related genes, and go together with by a large DHA-1- plasmid.
Barakat, G. (2021). SFO-1 and DHA-1 genes Detection in β-lactamases-producing Klebsiella pneumoniae Causing Blood Stream Infections Acquired in Intensive Care Units in Mansoura University Hospitals. Egyptian Journal of Medical Microbiology, 30(3), 43-51.
MLA
Ghada Barakat. "SFO-1 and DHA-1 genes Detection in β-lactamases-producing Klebsiella pneumoniae Causing Blood Stream Infections Acquired in Intensive Care Units in Mansoura University Hospitals". Egyptian Journal of Medical Microbiology, 30, 3, 2021, 43-51.
HARVARD
Barakat, G. (2021). 'SFO-1 and DHA-1 genes Detection in β-lactamases-producing Klebsiella pneumoniae Causing Blood Stream Infections Acquired in Intensive Care Units in Mansoura University Hospitals', Egyptian Journal of Medical Microbiology, 30(3), pp. 43-51.
VANCOUVER
Barakat, G. SFO-1 and DHA-1 genes Detection in β-lactamases-producing Klebsiella pneumoniae Causing Blood Stream Infections Acquired in Intensive Care Units in Mansoura University Hospitals. Egyptian Journal of Medical Microbiology, 2021; 30(3): 43-51.
)
View on SCiNiTO