The Identification of Microbial Pathogens Using Matrix Assisted Laser Desorption Ionization Time of Flight in Medical Intensive Care Unit Beni-Suef University Hospital
Document Type : New and original researches in the field of Microbiology.
General Directorate of Infection Control, Ministry of Health and Population
Abstract
Background: Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) is a novel technique for identification of microbes. This new method led to a new era in microbial identification because of its rapid, accurate, valid, simple and relatively decreased cost. Objectives: The aim of this study was identification of predominant pathogens by MALDI-TOF technique. Methodology: Pathogens were identified by both conventional methods and MALDI-TOF. Results: From July till December 2018, predominant pathogens were Klebsiella pneumoniae (21%), Pseudomonas aeroguinosa and Candida each constitutes (17%), E-coli (10%), Staph. aureus (9%), Acinetobacter (9%). Identification of isolates (from September to December 2018) by MALDI-TOF revealed a total agreement of (94.1%) with conventional method at genus level, (88.2%) at level of species. Kappa agreement revealed almost perfect correlation between both techniques. Conclusion: The MALDI-TOF results might suggest that its’ usage may be dependable for microbiological identification.
Mohamed, M. (2021). The Identification of Microbial Pathogens Using Matrix Assisted Laser Desorption Ionization Time of Flight in Medical Intensive Care Unit Beni-Suef University Hospital. Egyptian Journal of Medical Microbiology, 30(3), 65-69.
MLA
Marwa Nabil Mohamed. "The Identification of Microbial Pathogens Using Matrix Assisted Laser Desorption Ionization Time of Flight in Medical Intensive Care Unit Beni-Suef University Hospital". Egyptian Journal of Medical Microbiology, 30, 3, 2021, 65-69.
HARVARD
Mohamed, M. (2021). 'The Identification of Microbial Pathogens Using Matrix Assisted Laser Desorption Ionization Time of Flight in Medical Intensive Care Unit Beni-Suef University Hospital', Egyptian Journal of Medical Microbiology, 30(3), pp. 65-69.
VANCOUVER
Mohamed, M. The Identification of Microbial Pathogens Using Matrix Assisted Laser Desorption Ionization Time of Flight in Medical Intensive Care Unit Beni-Suef University Hospital. Egyptian Journal of Medical Microbiology, 2021; 30(3): 65-69.
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