Phenotypic Methods and Molecular Metallo-β-lactamases Genes Detection in Different Clinical Carbapenems Resistant Acinetobacters Isolates from Tanta University Hospitals
Document Type : New and original researches in the field of Microbiology.
Medical Microbiology and Immunology Department, Faculty of Medicine, Tanta University
Abstract
Background: Acinetobacter species are very common in hospital acquired infections; Carbapenems resistant species become common in hospital and represent a major health problem. Methodology: Our study was carried on 150 patients from ICU; we compared different phenotypic methods and PCR for detection of carbapenems resistance in Acinetobacters. Results: 88.9% of resistant Acinetobacters were detected by Kirby Bauer Disk diffusion method, 54.2%by Modified Hodge test, 70.8 % by EDTA disc synergy, 85.4% by Amp C disk test, 33.3% by PCR bla-IMP and 12.5% by PCR bla-VIM. Conclusion: We recommended for detection of carbapenems resistance in Acinetobacters the phenotypic methods as they are rapid, accurate methods and less costly than PCR. Note:bla-IMP gene (NCBI GenBank Nucleotide accession # LC103138.1); bla-VIM gene (NCBI GenBank Nucleotide accession # MT185944.1
GenBank accession number S71932
Eshra, K. (2021). Phenotypic Methods and Molecular Metallo-β-lactamases Genes Detection in Different Clinical Carbapenems Resistant Acinetobacters Isolates from Tanta University Hospitals. Egyptian Journal of Medical Microbiology, 30(3), 89-95.
MLA
Kareman Ahmed Eshra. "Phenotypic Methods and Molecular Metallo-β-lactamases Genes Detection in Different Clinical Carbapenems Resistant Acinetobacters Isolates from Tanta University Hospitals". Egyptian Journal of Medical Microbiology, 30, 3, 2021, 89-95.
HARVARD
Eshra, K. (2021). 'Phenotypic Methods and Molecular Metallo-β-lactamases Genes Detection in Different Clinical Carbapenems Resistant Acinetobacters Isolates from Tanta University Hospitals', Egyptian Journal of Medical Microbiology, 30(3), pp. 89-95.
VANCOUVER
Eshra, K. Phenotypic Methods and Molecular Metallo-β-lactamases Genes Detection in Different Clinical Carbapenems Resistant Acinetobacters Isolates from Tanta University Hospitals. Egyptian Journal of Medical Microbiology, 2021; 30(3): 89-95.
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