Medical Microbiology & Immunology Department, Faculty of Medicine, Zagazig University, Egypt
Abstract
Background: Hashimoto's thyroiditis (HT) is a T cell-mediated autoimmune disease that primarily affects females. IFN- γ is a critical cytokine that has been related to the pathogenesis of HT. Objectives: We aimed to evaluate serum and expression levels of interferon-gamma (IFN- γ) in Egyptian women with HT and to assess the association between serum and expression levels of IFN- γ with clinical and laboratory characteristics of HT. Methodology: This case-control study included 120 women with HT and 70 controls. IFN- γ mRNA expression was analyzed using real-time polymerase chain reaction. Serum IFN- γ was measured using enzyme-linked immunosorbent assay. Results: Serum IFN- γ level and the level of IFN- γ mRNA are both sensitive and specific to be used as diagnostic markers for HT with cut off values of 28.57 pg/ml and 3.55 respectively. Both showed a significant positive correlation with TPO-Ab and Tg-Ab, obesity indices, dyslipidemia, and TSH, while they have a negative correlation with FT3, FT4. Conclusions: Serum IFN- γ level and the level of IFN- γ mRNA are both sensitive and specific to be used as diagnostic markers for HT, significantly correlated with thyroid autoantibodies and thyroid function tests.
El Shabrawy, R. (2021). Expression profile of interferon-gamma (IFN- γ) mRNA as diagnostic molecular signatures of Hashimoto's Thyroiditis. Egyptian Journal of Medical Microbiology, 30(2), 117-123.
MLA
Reham M El Shabrawy. "Expression profile of interferon-gamma (IFN- γ) mRNA as diagnostic molecular signatures of Hashimoto's Thyroiditis". Egyptian Journal of Medical Microbiology, 30, 2, 2021, 117-123.
HARVARD
El Shabrawy, R. (2021). 'Expression profile of interferon-gamma (IFN- γ) mRNA as diagnostic molecular signatures of Hashimoto's Thyroiditis', Egyptian Journal of Medical Microbiology, 30(2), pp. 117-123.
VANCOUVER
El Shabrawy, R. Expression profile of interferon-gamma (IFN- γ) mRNA as diagnostic molecular signatures of Hashimoto's Thyroiditis. Egyptian Journal of Medical Microbiology, 2021; 30(2): 117-123.
)
View on SCiNiTO