Document Type : New and original researches in the field of Microbiology.
Authors
1
Drug Radiation Research Dep, Biotechnology Division, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Cairo, Egypt
2
Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt
3
Microbiology Dep, Biotechnology Division, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Cairo, Egypt
Abstract
Background: Taxol is a commercial anticancer drug, with broad spectrum towards different cancer cell as breast, lung, head and uterine cancers. Taxol producing by endophytes recovered from the medicinal plants. Objective: This evaluating of Taxol production by endophytes isolated from Moringa and Hibiscus plants. Enhancementof Taxol production by different types of media and Gamma irradiation were studied. The antimicrobial activity of Taxol was observed. Methodology: Fungal isolates were recovered from Moringa and Hibiscus plant. The samples were sterilized with ethanol (70%), wash with sterile water, sterilized tissue by sodium hypochlorite 2.5% then wash with sterile water. The isolates were grown on PDB media, after incubation, the cultures were filtered and characterized by UV-Vis., TLC, HPLC and FTIR. Results: Twenty seven isolates were separated from Moringa (16 isolates) and Hibiscus plant (11 isolates). These organisms were observed from barks (seven isolates), twigs (eight isolates), leaves (five isolates) and buds (seven isolates) of plants. Purified fungal isolates were Aspergillus, Penicillium, Cladosporium and Fusarium. Between these isolates, the prevalence of genus Aspergillus was detected (70.3%) while Fusarium remark to be (14.8%); also Penicillium was reported (11.1%) and finally Cladosporium (3.7%). From HPLC results the optimum Taxol production was Penicillium sp.5 (54.42µg/L), Aspergillus niger10 (43.95µg/L) and Fusarium sp.8 (26.8µg/L) on potato dextrose agar medium. Conclusion: Improvement of Taxol harvest by Penicillium sp.from (54.42µg/l) to (184.3µg/l) on Dox medium, maximum yield of Taxol was appeared at 1.25 kGy (274.6µg/l). Finally a significant antimicrobial of Taxol towards E. coli 15.0mm and Ent. cloacae 22.0mm.
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