Screening for Group B Streptococci in Pregnant women by culture and polymerase chain reaction methods

Document Type : New and original researches in the field of Microbiology.

Authors

1 Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, Egypt

2 Department of Obstetrics and Gynecology, Faculty of Medicine, Assiut University, Assiut, Egypt

3 Department of Oncological Clinical Pathology,South Egypt Cancer Institute, Assiut University, Assiut, Egypt

4 Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt

Abstract

Background: Group B Streptococcus (GBS) inhabits pregnant women's genitourinary tracts and spreads to the offspring. Objective: This study aimed to screen for GBS colonization in pregnant women, to compare between GBS culture, VITEK® 2 and PCR, and to assess the antimicrobial susceptibility of GBS isolates. Methodology: Vaginal and rectal swabs were collected from 82 pregnant women. GBS was isolated by culture on Strep B Select. Confirmation by VITEK® 2 and PCR for atr and 16S rRNA genes was done. Results: Sixteen vaginal (19.5%) and 22 rectal colonized cases (26.8%) were positive for GBS by culture on Strep B Select agar. GBS was detected in 10 vaginal (12.2%) and 16 rectal colonized cases (19.5%) by VITEK® 2 and PCR for both atr and 16S rRNA genes.  Conclusion: It is suggested that pregnant women should be screened for GBS colonization.  The best precise tests to detect GBS are PCR and VITEK® 2.

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