Document Type : New and original researches in the field of Microbiology.
Authors
1
Botany Department, Faculty of Science, Sohag University
2
Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University
3
Medicinal and Aromatic Plants Department, Director of Horticulture Research Institute, Agriculture Research Center, Giza
4
Researcher in Medicinal and aromatic plants department, Horticulture research institute, Agriculture research center, Giza
Abstract
Background: Helicobacter pylori is a micro-aerophilic Gram-negative bacteria. The increasing resistance between strains was emerged, resistance to such antimicrobial agents continues to be alarming worldwide. Objectives: to isolation of H. pylori by bacterial culture and evaluate the resistance rate of bacteria to antimicrobials and effect of medicinal plant extracts (A. sativum and C. carvi essential oil, Moringa oleifera, Trigonella foneum, Lawsonia inermis) on H. pylori strains and detection of their effective components.. Methodology: This study was done on stool samples collected from 260 patients attending gastroenterology department at Sohag and Assiut University Hospital, collected during the period from March 2019 to February 2020. The bacterial cultures were done for isolation of the pathogenic bacteria and detection of their antibiotic susceptibility by disc diffusion method and Minimum inhibitory concentration (MICs) determination and treatment of resistant strains with medicinal plant extracts as natural products and detection of their active constituents qualitatively and quantitatively. Results: 87.0% and 59.2% sensitive strains of H. pylori to A. sativum and C. carvi essential oil and 67.4%, 57.1%, 44.9% susceptible strains to T. foneum gracium, M. oleifera, L. inermis were detected. Flavonoids, alkaloids, tannins, phenols as phytochemicals were detected in Moringa oleifera, Trigonella foneum, Lawsonia inermis methanolic extracts causing their antibacterial activity. Disulfide diallyl (30.12%) and limonene (46.39%) and carvone (50.30%) were as bioactive compounds in Allium sativum and Carium carvi essential oil. Conclusion: results, we concluded that the medicinal plant extracts were the most effective agents as antimicrobials so we recommend the use of these extracts as alternatives in the future to treat the resistance problem.
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