The in Vitro Effect of Vitamin D on the Antibiotic Susceptibility of Carbapenem Resistant Pseudomonas species

Document Type : New and original researches in the field of Microbiology.

Authors

1 Medical Microbiology and Immunology, Cairo University

2 Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo, Egypt

Abstract

Background: Carbapenem-resistant Pseudomonas infections become a serious problem with limited options of treatment. Many studies support the role of vitamin D in competing different infections by many mechanisms through enhancing host immune defense. Objective: to assess the antimicrobial effect of vitamin D on carbapenem-resistant Pseudomonas. Methodology: Fifty carbapenem-resistant Pseudomonas isolates were studied. All isolates were tested for susceptibility to imipenem and meropenem using disk diffusion method. Modified carbapenem inactivation methods were done for carbapenemase and metallo-beta-lactamase detection. Isolates were tested for MIC of meropenem and vitamin D by broth-microdilution and their combination by checkerboard method. Results: Regarding the combination between meropenem and vitamin D; 10 isolates showed decrease in their MIC of meropenem. One isolate showed additive result, 5 isolates showed decrease more than 2 folds, 4 isolates showed 2 folds decrease. Conclusion: Vitamin D acts as an adjuvant to meropenem rather than having a direct antimicrobial effect on carbapenem-resistant Pseudomonas species.

Keywords

Main Subjects

Files

Share

How to cite

Statistics