Effect of subinhibitory concentrations of selected antibiotics and propolis on pyocyanin and biofilm production among Pseudomonas aeruginosa isolates in Alexandria, Egypt

Document Type : New and original researches in the field of Microbiology.

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Department of Microbiology and immunology, Faculty of Pharmacy, Pharos University in Alexandria, Alexandria, Egypt

Abstract

Background: Pseudomonas aeruginosa is a highly virulent microorganism that is implicated in various types of infections. It is armed with an arena of virulence factors that is mostly controlled by quorum sensing. The pigment pyocyanin and biofilm formation are of the important defense mechanisms used by the organism to establish infection. Objective: detection and quantification of biofilm mass and pyocyanin along with effect of subinhibitory concentrations of antibiotics on their magnitude. Methodology: In the present study, fifty Pseudomonas aeruginosa isolates were obtained from clinical laboratories from all over Alexandria governorate, Egypt. The isolates were tested for certain quorum sensing dependent virulence factors and the effect of subinhibitory concentrations of certain antibiotics, in addition  propolis extract, was assayed. Results: the sub MIC of selected antibiotics and propolis inhibited pyocyanin production. On the other hand, they had variable effects on the formed biofilm mass. Conclusion: the effect of sub inhibitory concentrations either on biofilm formation or pyocyanin production is very important to be tested and followed to predict bacterial behavior and assist in tailoring therapeutic regimen.

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