The Role of Lactobacillus against Pseudomonas aeruginosa infection caused by urethral catheter in Assiut Urology Hospital

Document Type : New and original researches in the field of Microbiology.

Authors

1 Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assuit, Egypt

2 Lecturer of Urology, Faculty of Medicine, Assiut University, Assuit, Egypt

3 Researcher in Microbiology and Immunology Department, Faculty of Pharmacy, Assuit University, Assuit, Egypt

Abstract

Background: The emergence of antibiotic-resistant Pseudomonas aeruginosa has renewed efforts to identify safe and natural substituent agents such as probiotics.  Objective: In the present study, we evaluated the role of the Lactobacillus acidophilus isolated from commercial capsules (Puritan Pride, USA) against Pseudomonas aeruginosa and its biofilm isolated from long-term urethral catheter. Methodology: Samples were collected from 120 patients admitted to Assuit Urology Hospital. Bacterial identification was done by cultivation on selective media and by biochemical tests. Antibiotic susceptibility Testing was performed. Biofilm assay was conducted by two methods: Congo red method and Microtiter plate assay. L. acidophilus was isolated and incubated anaerobically in MRS broth at 37ºC for 24h, then centrifugation for 15 minutes at 10000 rpm. The inhibition effect of probiotic on P. aeruginosa strains was carried out by two methods: Agar well diffusion and Microtiter plate assay. Results:  Only 37 out of 120 isolates were identified as P. aeruginosa. Antimicrobial susceptibility tests included 15 antibiotics; P. aeruginosa isolates showed multi-drug resistance for antibiotics. According to Microtiter Plate assay, 100% of isolates were biofilm forming. Antimicrobial effect of L. acidophilus using Agar well Diffusion Method showed 81% inhibition rate. The highest zone of inhibition by L. acidophilus was 25 mm. By Microtiter plate assay, Lactobacillus acidophilus was able to inhibit 91.8% of biofilm formed. Conclusions: Although biofilm produced by P. aeruginosa is hardly killed by various antibiotics, L. acidophilus isolated from commercial capsules has shown anti-biofilm activity that can be used as antimicrobial agent after appropriate in vivo testing.

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