Screening for Escherichia albertii in children with gastroenteritis in Pediatric Hospital at Assiut University

Document Type : New and original researches in the field of Microbiology.

Authors

1 Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, Egypt

2 Department of Oncological Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt

3 Gastroenterology and Hepatology Unit, Pediatric Department, Faculty of Medicine, Assiut University, Assiut, Egypt

4 Department of Microbiology and Immunology, Faculty of Pharmacy, Sphinx University, New-Assiut, Egypt

5 Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt

Abstract

Background: Escherichia albertii (E. albertii) was isolated from feces of the people suffering from gastroenteritis. It is problematic to differentiate between it and other species of Enterobacteriaceae.Objective: The work aimed to determine the prevalence of E. albertii in stool samples from the children with gastroenteritis and its relation to some clinical and demographic factors.Methodology: In this study, 296 fecal samples were cultivated on MacConkey, xylose lysine deoxycholate and Hektoen enteric agar plates. Vitek®2 was used to analyze the isolates. By PCR lysP, mdh and eae genes were detected for E. albertii. Results: Out of 296 samples, 35 isolates (11.8%) were identified asE. albertii.Younger children were more vulnerable. The gender was not a risk factor.Diarrhea was watery in all cases. Vomiting, abdominal pain and fever were not presented in all cases. Conclusion:  E. albertii can cause gastroenteritis. The use of PCR for precise diagnosis can be beneficial.

Main Subjects