Microbiology Department, Medical Research Institute, Alexandria University, Egypt
Abstract
Background: Biofilm and siderophores protect K. pneumoniae, making them resistant to antimicrobials and increase the ability to evade immune system. Objective: The aim of the present study was to detect some of the virulence genes responsible for biofilm formation and siderophore production implicated in the pathogenesis of K. pneumoniae. Methodology: Crystal violet method was used to detect biofilm phenotypically, hypermucoviscosity by string test and virulence genes were detected by PCR. Results: Phenotypically, biofilm formation was positive in 76% and negative in 24%. Genotypically, FimH and ycfm were detected in 100% of isolates while mrkD in 98%. Kfu, entB and iutA were found in 100%, 68% and 34% respectively. Phenotypically by string test 66% of isolates were classified as hypermucoviscous, while 34% as classical. rmpA gene was detected in 52% of isolates. Conclusion: FimH, mrkD, ycfm, Kfu and iutA genes are implicated in multisystemic infection of K. pneumoniae. Enterobactin gene is more predominant than other siderophore genes.
Naga, I. (2021). Detection of Biofilm and Siderophore Encoding Genes Implicated in the Pathogenesis of Klebsiella pneumoniae Isolated from Different Clinical Specimens. Egyptian Journal of Medical Microbiology, 30(1), 101-108.
MLA
Iman Salah Naga. "Detection of Biofilm and Siderophore Encoding Genes Implicated in the Pathogenesis of Klebsiella pneumoniae Isolated from Different Clinical Specimens". Egyptian Journal of Medical Microbiology, 30, 1, 2021, 101-108.
HARVARD
Naga, I. (2021). 'Detection of Biofilm and Siderophore Encoding Genes Implicated in the Pathogenesis of Klebsiella pneumoniae Isolated from Different Clinical Specimens', Egyptian Journal of Medical Microbiology, 30(1), pp. 101-108.
VANCOUVER
Naga, I. Detection of Biofilm and Siderophore Encoding Genes Implicated in the Pathogenesis of Klebsiella pneumoniae Isolated from Different Clinical Specimens. Egyptian Journal of Medical Microbiology, 2021; 30(1): 101-108.
)
View on SCiNiTO