Septic Arthritis: Microbiological Etiology and Molecular Detection of the Most Resistant Etiological Agents

Document Type : New and original researches in the field of Microbiology.

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Microbiology and Immunology Department, Faculty of Pharmacy, Pharos University in Alexandria, Alexandria, Egypt

Abstract

Background: Septic arthritis is a serious emergency causing remarkable morbidity and mortality worldwide. Expeditious diagnosis and effective treatment are necessary to achieve better clinical outcomes and avoid devastating joint consequences. Objective: we focused to detect the most common etiological agent and associated resistance to commonly used antibiotics in Nariman Hospital in Alexandria, Egypt. Molecular detection of mecA gene which causes resistance in methicillin-resistant Staphylococcus aureus and results in treatment failure was studied among our isolates. Methodology: One hundred and fifty joint fluid aspirates were included in the study. Identification of the isolates was done by conventional microbiological methods and BACTEC MGIT 960 TM system. Antibiotic susceptibility tests were done to detect resistance in our isolates. Molecular amplification of mecA gene was done in isolated methicillin-resistant Staphylococcus aureus (MRSA). Results: It was noted that most the of specimens were collected from males. Culture results showed monomicrobial bacterial growth in 90.2% of samples tested. Staphylococcus aureus was the major organism isolated. 88.9% of methicillin-resistant Staphylococcus aureus (MRSA) were positive for mecA gene. Conclusion: As far as we know, this is the first research in Alexandria investigating the most common etiological agent and associated resistance resulting in treatment failure in the leading orthopedic hospital in Alexandria.

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