Prevalence of Human Papilloma Virus and Adeno-associated Virus in Semen of Infertile Undergoing Assisted Reproduction

Document Type : New and original researches in the field of Microbiology.

Authors

1 Microbiology and Immunology Department, Faculty of Medicine, Mansoura University

2 Division of Andrology, Faculty of Medicine, Mansoura University

3 Mansoura Integrated Fertility Centre, Mansoura, Egypt

4 Department of Microbiology and Immunology, Faculty of Medicine, Mansoura University

Abstract

Background: HPV and AAV infection are suggested to be a risk factor for infertility in men. Objective: To detect the prevalence of human papilloma virus (HPV) and adeno associated virus (AAV) in semen of men suffering from infertility. Methodology: One hundred and thirty-eight Egyptian men were included in the study, 69 infertile patients and 69 fertile men. Conventional PCR was done for HPV genome determination, and nested PCR for AAV detection from the semen samples. Results: 23.2% infertile men samples were containing virus, compared with 4.3% positive semen samples among fertile men with a significant difference. Among infertile patients, 10.1% of the samples had HPV genome, 8.7% had AAV and 4.3% showed co-infection with both viruses. Regarding semen parameters, 52.2% of infertile patients showed normal parameters, 47.8% showed asthenozoospermia, oligoasthenoteratozoospermia (OAT) in 33.3%, oligoasthenospermia (OA) in 27.3 %, and oligozoospermia in 6.1%. All infertile patients underwent ICSI, neither of HPV, AAV or co-infection affected the success rate. Out of 16 patients positive for viral infection, 9 (56.3%) showed successful ICSI and 7 patients (43.8%) had failed outcome with nonsignificant difference. Conclusions: HPV and AAV must be excluded in male infertility, due to their high prevalence among this group with negative effects on semen parameters. 

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