Diagnosis of Helicobacter pylori infection in Assiut University Hospital and medical laboratories by using non-invasive tests

Document Type : New and original researches in the field of Microbiology.

Authors

1 Botany Department, Faculty of Science, Sohag University

2 Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University

3 Medicinal and Aromatic Plants Department, Director of Horticulture Research Institute, Agriculture Research Center, Giza

4 Researcher in Medicinal and Aromatic Plants Department, Horticulture Research Institute, Agriculture Research Center, Giza

Abstract

Background: Helicobacter pylori is a gram negative bacterium and considered as one of the causative factors of  gastritis and peptic ulceration.The exact route of transmission is still unknown. H. pylori infection was highly prevalent worldwide. Objectives:  to detect H. pylori infection rate by different tests including ELIZA tests for anti H. pylori antibodies (IgM, IgG, IgA) and H. pylori antigen, to isolate H. pylori by bacterial culture, to evaluate antimicrobial susceptibility of bacteria and to estimate bacterial infection rate according to clinical symptoms of patients. Methodology: This study was done on 130 stool samples and 130 blood samples collected from 130 patients attending Gastroenterology Department of Assiut University Hospital and some special medical laboratories, collected during the period from March 2019 to February 2020. Stool samples were processed to detect H. pylori antigen titers and to isolate H. pylori bacteria, blood samples were processed to detect anti H. pylori antibodies. Antimicrobial susceptibility of H. pylori was evaluated to ten antibiotics by disc diffusion method. Results: H. pylori infection rate was 86.1% by bacterial culture, 81.5% by anti-H. pylori IgG antibodies test , 48.5% by anti-H. pylori IgA antibodies test, 13.8% by anti-H. pylori IgM antibodies test, 76.9% by H. pylori antigen test. 39.2% of  H. pylori infected patients had abdominal pain. Bacterial strains were resistant to several antibiotics. Conclusion: we concluded that anti-H. pylori IgG antibodies and H. pylori antigen tests were the preferable tests for diagnosis of H. pylori infection. According to statistical analysis, these results were more reliable and valuable. Abdominal pain was the most common symptom in H. Pylori infection. ciperofloxacin and amoxicillin were the most effective antibiotics for inhibition of bacteria. 

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