Effect of Silver Nanoparticles on Biofilm Producing Multi Drug Resistant Uro-pathogenic E. coli isolated from Catheterized Patients in Mansoura University Hospital

Elbehairy, Eman Nashat; El Nagdy, Magda M.; Weefky, Ghada F.; Nabiel, Yasmin

doi:10.21608/ejmm.2022.211962

Effect of Silver Nanoparticles on Biofilm Producing Multi Drug Resistant Uro-pathogenic E. coli isolated from Catheterized Patients in Mansoura University Hospital

Document Type : New and original researches in the field of Microbiology.

Authors

¹ Microbiology and immunology department faculty of medicine mansoura university

² Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura, Egypt

³ Department of Anesthesia and Surgical ICU, Faculty of medicine, Mansoura university, Mansoura, Egypt

10.21608/ejmm.2022.211962

Abstract

Background:Uro-pathogenic E. coli (UPEC) is considered one of the members of the extra-intestinal pathogens, that predominantly cause urinary tract infections. Objectives: Assessment of the prevalence of MDR UPEC in catheterized patients admitted into Mansoura University Hospital, then detection of biofilm production by isolated MDR UPEC strains, and finally testing the effect of silver nanoparticles on biofilm producing ability of the isolated strains. Methodology: 155 urine samples were processed to detect E. coli strains, Antibiotic susceptibility profile of resulting E. coli isolates was determined by Kirby- Bauer disc diffusion method to select MDR UPEC strains, then detection of biofilm production using tissue culture plate method among isolated MDR UPEC strains, and finally evaluation of anti-biofilm activity of silver nanoparticles (AgNPs) by re-detection of biofilm formation after incubation with various concentrations of silver nanoparticles. Results: Out of these urine samples, 35 isolates (22.6%) revealed E. coli. Out of them, 34 isolates (97.1%) were MDR E. coli. 29 isolates (85%) of MDR UPEC were biofilm producers. The antibiofilm effect was observed in dose dependent manner. Silver nanoparticles with concentration of 100μg/ml, recorded maximum antibiofilm effect (63.22%) followed by concentrations of 75, 50, and 25μg/ml which were able to eliminate the biofilm formation of MDR UPEC on the plate surface by (59.91%), (55.24%), (43.61%) respectively.Conclusion: Silver nanoparticles play a major role in the inhibition of biofilm formation by MDR UPEC.
Abbreviations: (AgNPs, Silver nanoparticles; CAUTI, Catheter associated urinary tract infection; MDR, Multidrug resistance; TCP, Tissue culture plate; TSB, Trypticase soy broth; UPEC, Uro-pathogenic E. coli).

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