Prevalence of Acquired Colistin Resistance among Gram Negative Bacilli Isolated from Patients Admitted at Cairo University Hospitals

Sorour, Ashraf E.; Ibrahim, Karim Abdo Abdo; Hegab, Asmaa

doi:10.21608/ejmm.2022.211995

Prevalence of Acquired Colistin Resistance among Gram Negative Bacilli Isolated from Patients Admitted at Cairo University Hospitals

Document Type : New and original researches in the field of Microbiology.

Authors

¹ Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Egypt

² Deartment of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo11562, Egypt

10.21608/ejmm.2022.211995

Abstract

Background: Polymyxins were mostly ignored few decades ago because of their toxicity. Now are considered as a last-line therapy to treat infections caused by multidrug resistant Gram-negative bacilli. Colistin resistance mediated by chromosomal mutations and more recently by plasmid‐borne mcr genes, is increasingly being reported in different countries. Objective: The aim of the present study is to determine the prevalence of acquired colistin resistance among gram negative bacilli isolates as well as to compare colistin resistance among different Gram-negative bacilli spp. It also aimed to study the antibiotic susceptibility of different Gram-negative bacilli isolates. Methodology: A total of 180 different clinical specimens were collected from the patients admitted at different Departments of Cairo University Hospitals until 115-Gram negative bacilli isolates were isolated. Results: The prevalence of acquired colistin resistance among gram negative bacilli isolates was found to be 10.4% (12/115). The prevalence of colistin resistance among different Gram-negative bacilli isolates was as follows: 12.5% among E. coli (3/24), 9.5% among Klebsiella spp. (4/42), 13.8% among Pseudomonas spp. (4/29) and 5.0% among Acinetobacter spp. (1/20). The overall colistin resistance rate among Enterobacteriaceae was 10.6% (7/66) while the overall colistin resistance rate among gram negative bacilli was 10.4% (12/115). Conclusions: Two-thirds (66.7%) of colistin resistant Gram-negative bacilli isolates were isolated from ICUs compared to other departments. All colistin resistant Gram-negative bacilli isolates were isolated from hospital acquired infections.

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