Detection of Exotoxin a Gene in Pseudomonas aeruginosa Strains Isolated from Burn Infections in Tanta University Hospital

Document Type : New and original researches in the field of Microbiology.

Authors

1 medical microbiology and immunology , faculty of medicine, Tanta university

2 Department of Surgery and burn Faculty of Medicine, Tanta University, Egypt

Abstract

Background: Multi-Drug Resistant (MDR) P. aeruginosa is a major health problem in burn infection. Exotoxin A gene plays an important role in pathogenesis of burn infections Objective: the isolation of MDR p.aeruginosa from infected burns and Detection of Exotoxin-A (Exo-A) gene in MDR P. aeruginosa isolates using conventional Polymerase Chain Reaction (PCR). Methods: The study was carried out on 200 samples of burn patients in a form of burn swabs, these patients admitted to Burn Units at Tanta university Hospitals. PCR was done on isolated MDR P.aeruginosa for detection of exotoxin- A gene. Results: P. aeruginosa was the most common organism isolated from burn infection with a percentage of 32.5% .96.3% of them contained the EXO-A gene while only two cases (3.3%) didn't contain the gene. Conclusion: EXO-A gene is an important virulence factor of P.aeruginosa infected burns. MDR P. aeruginosa incidence increased in hospitals.

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