Evaluation of OXA-48 in Carbapenem Resistant Gram-Negative Isolates

Document Type : New and original researches in the field of Microbiology.

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Microbial Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt.

Abstract

Background: Carbapenems production represents the most important mechanism that affects carbapenem activity. Among the Enterobacteriaceae, three molecular types of carbapenems are of major clinical importance since they are frequently associated with severe nosocomial infections. These are KPC (Ambler class A), NDM (class B) and OXA-48 (class D). Objective: The aim of the work is to determine the prevalence of carbapenem resistance and carbapenems encoding genes among clinical K. pneumoniae isolates obtained from patients at Intensive Care Units (ICUs) of Mansoura Oncology Institute. Methodology: This study included twenty-four Carbapenem resistant gram-negative bacilli (CRG), samples were collected from the Oncology Center in Mansoura University, Egypt, between October 2014 and February 2017. Samples were collected from different body sites such as: swabs, blood, urine, pus aspirate. Results: OXA-48 gene was positive in 16 samples and negative in 8 samples. Among the OXA-48 positive samples, 8 samples were positive by Modified Hodge test- Ertapenem disk, 5 samples were positive by Modified Hodge test- Meropenem disk, 3 samples were positive by  combined disk test-Meropenem boronic acid Class A and 5 samples were positive by combined disk test- Imipenem EDTA Class B. Among the OXA-48 negative samples, 2 samples were positive by Modified Hodge test- Ertapenem disk, 3 samples were positive Modified Hodge test- Meropenem disk and 3 samples were positive by combined disk test- Imipenem EDTA Class B. Conclusion: Antibiotic resistance is considered a global health problem which is caused by misuse of antibiotics with the lack of the new antibiotic’s development.

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