Document Type : New and original researches in the field of Microbiology.
Authors
1
clinical pathology department,faculty of medicine, Beni-Suef university
2
Internal Medicine Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt Taibah University, King Saudi Arabia
3
Tropical Medicine Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt
4
Internal Medicine Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt
5
Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt
Abstract
Background: Hepatocellular carcinoma (HCC) is the most prevalent malignancy affecting developing populations Aflatoxin B1 (AFB1), an established carcinogen produced by certain strains of fungi, particularly Aspergillus parasiticus and Aspergillus flavus. The carcinogenic effects of AFB1 in hepatocellular carcinoma (HCC) are ascribed to AFB1-DNA adduct formation with p53 gene mutation. Objective: The present study aimed was to investigate the prevalence of single nucleotide polymorphism (SNP) in rs 25487 of X-ray cross complementing group 1 protein (XRCC1) in association with AFB1 in patients with hepatocellular carcinoma (HCC). Methodology: The study included 100 patients with HCC and 100 healthy control subjects. Blood samples were withdrawn from each subject and SNP in rs 25487 XRCC1 was estimated by real time polymerase chain reaction (PCR) and determination of blood levels of AFB1. Results: There was a dramatic increase in the level of aflatoxin B1 in patients (1.02±0.7ng/gm) compared to control subjects (0.8±0.6ng/gm), P=0.01. A significant increase in mutant genotypes CT and TT was found in HCC patients (43% and 7%, respectively) compared to control subjects (27% and 1%, respectively), with a notable increase in the dominant genotype CC in healthy controls (72%) compared to patients with HCC (50%), P=0.002. There was also a statistically significant increase in C allele (85.5%) in the control subjects compared to patients with HCC (71.5%) with a significant increase in T allele in patients (28.5%) compared to control subjects (14.5%), P=0.001. Furthermore, a significant difference in level of aflatoxin B1 was detected in patients with different genotypes of rs25487 XRCC1, with high levels of aflatoxin B1 found in patients with CT genotype (1.2±0.5), P=0.002. Conclusion: This study revealed the relationship of SNP of XRCC1 rs25487 with hepatocellular carcinoma, showing a significant association between this SNP and the increased level of aflatoxin B1 in patients with HCC.
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