Evaluation of the Antimycotic activity of Terconazole proniosomal Gel

Bahy, Rehab; Helal, Doaa Ahmed

doi:10.21608/ejmm.2022.229668

Evaluation of the Antimycotic activity of Terconazole proniosomal Gel

Document Type : New and original researches in the field of Microbiology.

Authors

Rehab Bahy ¹
Doaa Ahmed Helal ²

¹ Microbiology and Immunology Faculty of Pharmacy Fayoum university- Fayoum-Egypt

² Department of pharmaceutics, Faculty of Pharmacy, Fayoum University, Fayoum, Egypt.

10.21608/ejmm.2022.229668

Abstract

Background: Terconazole (TCZ) counted is an antifungal drug with a broad spectrum of activity against Candida species. TCZ has been shown to be more active than imidazoles. Objectives: The aim of this research was to assure the potential of proniosomalgelfor the topical delivery of terconazole, and to detect its MIC against Candida albicans comparing it with the reported MIC of terconazole cream 0.8%. Methodology: The coacervation phase separation process was used to develop Terconazole-loaded proniosomal gels. The particle size (PS) and entrapment efficiency percentage of the produced proniosomal gels were assessed and in-vitro drug release. The particle size varies from (17.06–to 48.1 nm), range of EE% is (83.41%–95.66%). F4 (span 60, tween 80 (1:1), and cholesterol) have the highest EE% (95.66%), was tested by Transmission electron microscopy (TEM) and a zeta potential (ZP) test were performed. Results: The outcomes revealed a nanosized spherical vesicleofterconazole. Proniosomal gel MIC was (32µg/ml) lower than the reported MIC of market terconazole cream 0.8% (128µg/ml). Conclusion: Proniosomal gel achieved the desired sustained topical effect.There was no significant change in EE percent, PS, or ZP in physical stability investigation.

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