Background: Bloodstream infections are serious ailments, that require prompt appropriate treatment. This is challenging with the growing number of multi-drug resistant organisms. Objective: The aim of this study was to genotypically characterize the carbapenem resistance among cephalosporin resistant Enterobacteriaceae isolates causing bloodstream infections. Methodology: Twenty-eight enterobacterial isolates resistant to ceftazidime and cefepime causing bloodstream infections were collected. Their identification was confirmed using Vitek-2 system (Biomerieux, France), and their susceptibility pattern was assessed using disk diffusion method. They were genotypically tested for the presence of blaSHV, blaCTX-M as well as blaTEM. Furthermore, carbapenem resistant isolates were genotypically examined to detect blaKPC, blaIMP, blaNDM-1, blaVIM and blaOXA-48 genes. Results: The enterobacterial isolates were identified as Klebsiella pneumoniae and Escherichia coli. Of these, (85.71%) were carbapenem resistant. Genotypically blaCTX-M, blaSHV and blaTEM were detected in (85.71%), (89.29%) and (64.29%) of the twenty-eight isolates, respectively. Moreover, (66.67%) and (62.50%) of the carbapenem resistant isolates possessed blaOXA-48 and blaNDM-1, respectively. Only one harbored blaVIM. Moreover, blaIMP and blaKPC were not detected among these isolates. Ten (35.71%) of the isolates harbored beta-lactamases genes belonging to three beta-lactamases classes (A, B and D). Conclusion: The co-occurrence of different beta-lactamases belonging to diverse classes and the co-existence of blaOXA-48 and blaNDM-1 among isolates causing bloodstream infections is quite alarming and necessitates prompt action.
ElBaradei, A. (2022). Co-occurrence of blaNDM-1 and blaOXA-48 among carbapenem resistant Enterobacteriaceae isolates causing bloodstream infections in Alexandria, Egypt. Egyptian Journal of Medical Microbiology, 31(3), 1-7. doi: 10.21608/ejmm.2022.247169
MLA
Amira ElBaradei. "Co-occurrence of blaNDM-1 and blaOXA-48 among carbapenem resistant Enterobacteriaceae isolates causing bloodstream infections in Alexandria, Egypt". Egyptian Journal of Medical Microbiology, 31, 3, 2022, 1-7. doi: 10.21608/ejmm.2022.247169
HARVARD
ElBaradei, A. (2022). 'Co-occurrence of blaNDM-1 and blaOXA-48 among carbapenem resistant Enterobacteriaceae isolates causing bloodstream infections in Alexandria, Egypt', Egyptian Journal of Medical Microbiology, 31(3), pp. 1-7. doi: 10.21608/ejmm.2022.247169
VANCOUVER
ElBaradei, A. Co-occurrence of blaNDM-1 and blaOXA-48 among carbapenem resistant Enterobacteriaceae isolates causing bloodstream infections in Alexandria, Egypt. Egyptian Journal of Medical Microbiology, 2022; 31(3): 1-7. doi: 10.21608/ejmm.2022.247169
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