Association between Interleukin-6 Promoter Polymorphism (-174 G/C), Serum Interleukin-6 Levels and severity of sepsis

Document Type : New and original researches in the field of Microbiology.

Authors

1 Microbiology and immunology department faculty of medicine benha university

2 rehumatology and rehabilitation benha faculty of medicine benha university

3 Department of Medical Microbiology and Immunology, Faculty of Medicine, Benha University

Abstract

Background: Sepsis is a complicated clinical syndrome resulting from inflammatory reactions to bacteria or their products.  IL-6 is a potent inflammatory cytokine produced by many cells, such as leucocytes, monocytes, endothelial cells, and fibroblasts.  IL-6 plays an important role in the immune response and inflammation reactions   regulation. Objective: the present study aimed to detect if IL-6 serum level and polymorphism correlate with severity of sepsis.  Methodology: The study includes 70 subjects, 50 patients (Case) were chosen with at least 2 SIRS criteria and 20 healthy who were age matched.  The patients were categorized into systemic inflammatory response syndrome (SIRS), sepsis, severe sepsis, septic shock and multi-organ dysfunction syndrome (MODS) according to ACCP/SCCM. Bactec Culture on automated blood culture system (Bact/ALERT 3D) was used as a  gold standard method to prove sepsis .  Serum IL-6 was measured by Enzyme linked immunosorbent assay (ELISA). Genotyping of the IL-6–174 G/C promoter polymorphism was performed using restriction fragment length polymorphism (RFLP) analysis. Results: Our study reported a statistically high significant difference of   the IL-6 values between the case and control groups where p<0.001 and there was increasing values of serum IL-6 parallel with the developing  stages of sepsis. Also, the IL-6 values in the bacteriological culture positive and negative groups showed  differences  of statistically significant ratios (p<0.05 ).  Conclusion: Our findings reported a significant association between IL-6 promoter polymorphism (-174 G/C), levels of IL6  and severity of sepsis . 

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