Detection of Enterococcal asa1 and vanA Genes in Clinical Samples from Adult Immunocompromised Patients

Document Type : New and original researches in the field of Microbiology.

Authors

1 Department of Clinical Pathology

2 Department of Internal Medicine Faculty of Medicine – Beni-Suef University, Beni-suef, Egypt

3 Department of Microbiology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt

4 Department of Medical Microbiology and Immunology, Aswan Faculty of Medicine, Aswan, Egypt

Abstract

Background: Vancomycin resistant Enterococci (VRE) is important opportunistic nosocomial pathogens due to rapid spread, and limited treatment options. Objectives: The aims of this study were to assess incidence of enterococcal infections and VRE in adult immunocompromised patients and detection of asa1 and vanA genes among these isolates. Methodology: The study included 80 adult immunocompromised patients admitted to Beni-Suef University Hospital. Antimicrobial susceptibility was done by disc diffusion method then MIC was done for vancomycin resistant strains and Enterococcus was considered VRE if the MIC was ≥ 16μg/ml. PCR was done for detection of vanA and asa1 genes. Results: Enterococci were detected in 36 samples, 22.2% of isolates were vancomycin resistant. vanA and asa1 were detected in 33.3% and 63.8% of isolates respectively. VanA gene was detected in 4 (50%) of VRE whereas vanA gene was detected in 4 VSE isolates. Comclusion: Further studies are needed for detection of other virulence and resistance genes and their role in bacterial pathogenicity.

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