Carpabenem Resistant Klebsiella Species in Neonatal ICU in Beni-Suef University Hospital: Molecular Characterization and In-vitro Efficacy of Synergistic Antibiotic Combinations

Document Type : New and original researches in the field of Microbiology.

Authors

1 Department of Microbiology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt

2 Department of Clinical and Chemical pathology, Faculty of Medicine - Beni -Suef University, Beni-Suef, Egypt

3 Department of Clinical and Chemical pathology - Faculty of Medicine - Cairo University, Cairo, Egypt

Abstract

Background: Carbapenem resistant Klebsiella species [CRK] is increasing worldwide that may necessitate the use of combination therapy of antibiotics. Objectives: our aim was to characterize the main mechanisms of resistance carried by CRK isolates and to investigate the in-vitro efficacy of antibiotic combinations. Methodology: One hundred Klebsiella isolates were used for detection of KPC by modified Hodge test and boronic acid combination disc. PCR was done for detection of (OXA-48, KPC, OMP-35 and OMP-36) genes. The efficacy of antimicrobials combination against these isolates was also tested. Results: By boronic acid combination disc 62% were positive, by modified Hodge test 12% were positive. By PCR; 18% of isolates were positive for OMP-35, 16% for KPC, 14% OXA-48 and 6% for OMP36 genes. Antibiotic combination results showed that 64 and 26 isolates were sensitive to gentamicin- ciprofloxacin, meropenem-ciprofloxacin combinations respectively. Conclusion: We concluded that OXA-48, KPC, OMP-35 and OMP-36 genes were circulating in our hospital. Gentamycin and ciprofloxacin Combination may be effective.

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