Detection of Class-D OXA Carbapenemase Genes among Biofilm and Non-Biofilm Forming Acinetobacter baumannii Isolated from Suez Canal University Hospitals

Document Type : New and original researches in the field of Microbiology.

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Department of Medical Microbiology and Immunology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

Abstract

Background: Acinetobacter baumannii is recognized as opportunistic pathogen of increasing relevance in healthcare-associated infections. The emergence of carbapenemases genes in MDR-AB forming biofilm complicated treatment of infected patients with this microorganism. This study aimed to assess the prevalence of blaOXA genes genes among biofilm and non-biofilm forming A. baumannii. Methodology: forty seven A. baumannii strains were isolated and identified by conventional microbiological tests and API 20NE assay. The presence of blaOXA genes were studied by polymerase chain reaction. Biofilm formation was detected by microtiter plate method. Results: The highest percentage of resistance was to imipenem (85.1 %). 80.9 % of the isolates were resistant to ceftazidime, cefotaxime and meropenem. Thirty five A. baumannii isolates (74.5 %) were found to be MDR. Thirteen A. baumannii isolates (27.6%) were strong biofilm producers, 10 strains were moderate biofilm producers and 24 strains were weak or non-biofilm producers. blaOXA-23 was detected in 16 isolates (34.04 %), blaOXA-51 was detected in 8 isolates (17.02 %) and blaOXA-58 in 18 isolates (38.3%). None of the study isolates harbored blaOXA-24.Conclusion: Coexistence of the blaOXA genes along with biofilm formation, in MDR-AB strains, complicated treatment of infected patients with these bacteria.

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