Background: Gut microbiome could have central role in development of type 1 and type 2 diabetes mellitus (T1D) & (T2D). Objectives: The aim of the present study is to detect the composition of gut microbiota among T1D and T2D patients compared to healthy individuals. Methodology: The intestinal microbial composition of diabetic and heathy groups was investigated by both traditional culture techniques and polymerase chain reactions with amplification of the 16-23S rRNA intergenic spacer (IS) region. Results: By applying culture, diabetic groups showed a non-significant higher Firmicutes/ Bacteroidetes ratio (4.7% in T1D and 9% in T2D) compared to control group (4%) P value is 0.2058. While, PCR method showed that the diabetic group had a non-significant higher percentage of Lactobacillus spp., Faecalibacterium prausnitzii, Alistipes spp., Bacteroides thetaiotaomicron and Akkermansia muciniphila compared to healthy group (P value=0.407). Conclusion: Both diabetic groups showed a non-significant Firmicutes/Bacteroidetes ratio compared to control group by both culture and PCR techniques.
El Menofy, N., Radwan, H., & Radwan, S. (2020). The Diversity of Gut Microbiota among Type 1 and Type 2 Egyptian Diabetic Patients. Egyptian Journal of Medical Microbiology, 29(2), 123-130. doi: 10.21608/ejmm.2020.250216
MLA
Nagwan G. El Menofy; Hend M. Radwan; Sahar M.R. Radwan. "The Diversity of Gut Microbiota among Type 1 and Type 2 Egyptian Diabetic Patients", Egyptian Journal of Medical Microbiology, 29, 2, 2020, 123-130. doi: 10.21608/ejmm.2020.250216
HARVARD
El Menofy, N., Radwan, H., Radwan, S. (2020). 'The Diversity of Gut Microbiota among Type 1 and Type 2 Egyptian Diabetic Patients', Egyptian Journal of Medical Microbiology, 29(2), pp. 123-130. doi: 10.21608/ejmm.2020.250216
VANCOUVER
El Menofy, N., Radwan, H., Radwan, S. The Diversity of Gut Microbiota among Type 1 and Type 2 Egyptian Diabetic Patients. Egyptian Journal of Medical Microbiology, 2020; 29(2): 123-130. doi: 10.21608/ejmm.2020.250216
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