Background: bacteria have acquired the ability to adapt to stress conditions and developed various mechanisms of resistance; there is a need to develop new bactericidal materials. Objective: The aim of this study is to test the effect of silver nanoparticles (AgNPs) on increasing the antibiotic-susceptibility of MDR biofilm-producing Pseudomonas aeruginosa. Methodology: The antibiotic susceptibility testing of the isolates were done by the Modified Kirby- Bauer method and phenotypic detection of biofilm –forming isolates was done by the Congo Red Agar method. Results: Biofilm-production was found in16.3% of isolates and 77.6% were negative. The highest resistance rate was to piperacillin (67%), followed by ticarcillin-clavulanate (63%), while the isolates were highly sensitive to colistin (73%), polymyxin B (64%), and moderately-sensitive to meropenem (58%). Ten μL of Silver nanoparticles suspensions with size ranged from 20- 40 nm and average size 30 nm were added to the used antibiotics with retesting the isolates for their antibiotic susceptibility. Conclusion: AgNPs exhibited a strong synergistic effect with antibiotics through the significant increase of the antibiotic inhibitory zones and the improvement of the susceptibility profile of the isolates.
Thabet, A. (2020). Antibacterial action of silver nanoparticles on biofilm producing multidrug resistant Pseudomonas aeruginosa strains. Egyptian Journal of Medical Microbiology, 29(2), 145-152. doi: 10.21608/ejmm.2020.250238
MLA
Asmaa Nasr Eldin Thabet. "Antibacterial action of silver nanoparticles on biofilm producing multidrug resistant Pseudomonas aeruginosa strains". Egyptian Journal of Medical Microbiology, 29, 2, 2020, 145-152. doi: 10.21608/ejmm.2020.250238
HARVARD
Thabet, A. (2020). 'Antibacterial action of silver nanoparticles on biofilm producing multidrug resistant Pseudomonas aeruginosa strains', Egyptian Journal of Medical Microbiology, 29(2), pp. 145-152. doi: 10.21608/ejmm.2020.250238
VANCOUVER
Thabet, A. Antibacterial action of silver nanoparticles on biofilm producing multidrug resistant Pseudomonas aeruginosa strains. Egyptian Journal of Medical Microbiology, 2020; 29(2): 145-152. doi: 10.21608/ejmm.2020.250238