Multi-drug Resistance Profile of Virulent Biofilm forming Acinetobacter baumanii isolates in Intensive Care Units

Document Type : New and original researches in the field of Microbiology.

Authors

1 Faculty of Medicine Menofia University

2 Asmaa S. Sleem lecturer of medical microbiology and immunology, faculty of medicine, Menoufia university

3 Medical Microbiology and Immunology Department, Faculty of Medicine, Menoufia University, Egypt

Abstract

Background: Nosocomial infection with multidrug resistant biofilm forming Acinetobacter baumanii is a serious problem in healthcare settings with worldwide concern. Objectives: To study the prevalence and antimicrobial resistance profiles of Acinetobacter baumanii isolates detect biofilm formation and correlate biofilm FimH and bap genes with drug resistance. Methodology: This was a retrospective study performed in medical microbiology and immunology department, faculty of medicine, Menoufia university hospitals. Acinetobacter baumannii were isolated from intensive care units and phenotypically tested for biofilm formation by modified congo-red method. Antimicrobial susceptibility was evaluated by disc diffusion methods according to CLSI guidelines. Virulence genes (FimH and bap) concerned with biofilm formation were screened using polymerase chain reactions. Results: A total of 50 Acinetobacter isolates in ICUs were collected from 215 different clinical samples (23.3%). MDR was detected in 20 isolates and more than 28 isolates had formed biofilms. Both fimH & bap genes were detected by 58% and 30% combination rate with a significant correlation with virulence and drug resistance profiles. Conclusion: MDR Acinetobacter in ICUs has greater pathogenicity potentials emphasizing the importance of early detection using available laboratory techniques.

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