Characterization of β-Lactamase Genes among Multidrug- and Extensively Drug-Resistant Acinetobacter and Enterobacteriaceae Species Causing Infections in Critically Ill Patient

Document Type : New and original researches in the field of Microbiology.

Authors

1 Medical Microbiology and Immunology, Faculty of Medicine, Ain Shams University

2 Medical Department, King Abdulaziz Hospital, Jeddah, Saudia Arabia

3 Laboratory Department, King Abdulaziz Hospital, Jeddah, Saudi Arabia

4 Clinical Pathology Department, Faculty of Medicine, Al Azhar University, Egypt.

5 Infection Control Department, King Abdulaziz Hospital, Jeddah, Saudi Arabia

6 Surgery Department, King Abdulaziz Hospital, Jeddah, Saudi Arabia

Abstract

Background: Multidrug resistant infection is the leading cause of ICU morbidity and mortality. Gram-negative bacilli are the most prevalent pathogens with Acinetobacter being the most resistant. Objectives: Phenotypic and genotypic characterization of multidrug- and extensively drug-resistant Acinetobacter and Enterobacteriaceae species causing infections in critically ill patients with detection of targeted β-lactamase genes and its impact on patients' morbidity and mortality. Methodology: Antibiotic susceptibility testing was done. Genes for class A and class B β-lactamases were detected by multiplex polymerase chain reaction (PCR) and 5 carbapenemases genes were detected by real time PCR using GeneXpert Carba-R system. Results: 16.6 % of A. baumannii were MDR, 50% were XDR, 50% of K. pneumoniae were XDR and 100% of P. stuartii were XDR. Detected genes included blaSHV, blaCTX-M, blaGIM-1, blaSIM-1, blaOXA-48 and blaNDM-1. Conclusion: MDR/XDR infections had a significant impact on patients’ mortality. Molecular epidemiology is crucial to guide infection control committees about preventive measures that can contain MDR/XDR infections and prevent their detrimental effect on patients’ outcome.

Keywords

Main Subjects

Files

Share

How to cite

Statistics