Expression of Autophagy Related Genes mTOR, ATG10 and P62 in The Peripheral Blood Mononuclear Cells of Systemic Lupus Erythematosus Egyptian Patients

Abd Rhaman, Ekram Mahmoud; Abu Alfadl, Essam Mohammed; Mohammed, Dina Hamada; Sheneef, Abeer

doi:10.21608/ejmm.2023.277792

Expression of Autophagy Related Genes mTOR, ATG10 and P62 in The Peripheral Blood Mononuclear Cells of Systemic Lupus Erythematosus Egyptian Patients

Document Type : New and original researches in the field of Microbiology.

Authors

¹ medical micobiology and immunology faculty of medicine sohag university

² Rheumatology and Rehabilitation Faculty of Medicine, Sohag University

³ Medical microbiology and immunology Department. Faculty of Medicine Sohag University

⁴ Medical Microbiology and Immunology Department, Faculty of Medicine, Sohag University

10.21608/ejmm.2023.277792

Abstract

Background: Autoimmune diseases are knowen to be associated with abnormalities in autophagy. Objectives: In our study we evaluated the expression of autophagy related genes mTOR, P62 and ATG10 in the peripheral blood mononuclear cells (PBMCs) of systemic lupus erythematosus (SLE) and assess their relationship with disease activity and immunologic features. Methodology: The expression of mTOR, P62and ATG10 was detected by RT-PCR in 70 SLE subjects and 30 age- and sex-matched healthy controls. Data regarding demographics and clinical parameters were collected. Disease activity of SLE was evaluated according to the SLE Disease Activity Index (SLEDAI) score. Mann-Whitney test was used to analyze the expression of mTOR, P62 and ATG10 in the two groups. Spearman’s correlation was performed to analyze their relationship with disease activity and immunologic features. Results: The median and IQ (interquartile range) levels of P62 and ATG10 mRNA were significantly higher in SLE patients than the controls 1.11, (0.34-8.59) vs 0.25, (0.01-1.0) for P62 with P<0.002; 2.24 (0.55-19.31) vs 0.77 (0.02-1.0) for ATG10 with P<0.000). However, the mRNA relative expression level of mTOR in PBMCs was significantly very high in controls than cases of SLE 1.0, (1.0-2.14) vs0.31 ,(0.14-0.89) with P< 0.000). Conclusion: Correlation analysis showed that mTOR mRNA levels only correlated negatively with SLEDAI; also our research measure serum level of IL17 in the study population of cases and controls.

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