Role of MICA rs2596542 and IFN-gamma rs2069727 Polymorphism in HCC Development in Egyptian Patients with HCV-related HCC

Document Type : New and original researches in the field of Microbiology.

Author

Shebin Elkom National liver institute

Abstract

Background: Chronic infection with hepatitis C virus (HCV) is considered as a predisposing factor for liver cirrhosis (LC) and hepatocellular carcinoma (HCC). In several malignancies, MICA and IFN-γ genes were found to be highly expressed. Objectives: to identify the impacts of MICA rs2596542 and IFN-γ rs2069727 polymorphisms on LC and HCC development among Egyptian HCV patients, as well as impacts of gene-gene interaction on the disease incidence. Methodology: Blood samples were taken from 75 subjects with confirmed chronic hepatitis C (CHC), HCV-related LC or HCC on top of HCV infection and 25 healthy controls. Genotyping of MICA rs2596542 and IFN-γ rs2069727 polymorphisms were studied using real time PCR and PCR-Restriction fragment length polymorphism (RFLP) respectively. Results: A significantly elevated MICA rs2596542 TT genotype and T allele was found in HCC group (28%) and LC group (28%) compared to control group (16%). CC genotype and C allele were found to be more prevalent in healthy controls (64%) than HCC group (16%) and LC group (24%). AA genotype of IFN-γ rs2069727 SNP and A allele were significantly elevated in control group, while GG genotype and G allele were significantly elevated in HCC and LC groups. In assessing the hazard of HCC development, wild forms of both genes were higher in controls, and this is the protective forms while the mutant forms were elevated in HCC group. Conclusion: MICA rs2596542 and IFN-γ rs2069727 SNPs had significantly implicated in LC and HCC susceptibility.

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