Association between Single-nucleotide Polymorphism of miR-146a and Psoriasis

Tabl, Hala A.; Sabry, Hanan H.

doi:10.21608/ejmm.2019.282405

Association between Single-nucleotide Polymorphism of miR-146a and Psoriasis

Document Type : New and original researches in the field of Microbiology.

Authors

Hala A. Tabl ¹
Hanan H. Sabry ²

¹ Medical Microbiology & Immunology Department Faculty of Medicine, Benha University

² Dermatology, Venereology and Andorology Department, Faculty of Medicine, Benha University, Egypt

10.21608/ejmm.2019.282405

Abstract

Background: Micro-RNAs (miRNAs) play important roles in the development of different inflammatory conditions of the skin. However, few studies investigated the association between miR-146a and psoriasis. Aim of the work: This work aimed to study the association between miR-146a single-nucleotide polymorphism (SNP) rs2910164 and the risk of psoriasis and to study the association between levels of skin expression of miR-146a and the response to treatment. Methodology: The study was carried out on 120 patients with psoriasis and 60 control subjects. The miR-146a rs2910164 SNP was detected by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Levels of skin expression of miR-146a were detected by Real Time PCR before and 3 months after treatment with PUVA therapy. Results: Non significant association could be found between combined (CG+GG) genotypes and risk of psoriasis [OR (95% CI) = 0.81 (0.41-1.60)], (P=0.52). The level of miR-146a expression was significantly increased in patients compared to controls, with the highest level recorded in patients with CC genotype. There was a significant decrease in the miR-146a levels after treatment in both CC (P<0.001) & CG (P=0.01) genotypes but not in GG genotype (P=0.29). MiR-146a levels were higher in patients responding to treatment than non-responders (P<0.001). Also, there was significantly higher response to PUVA treatment in CC genotype (100%) than in CG genotype (50%) and GG genotype (20%) (P<0.001). In conclusion, no significant association could be found between combined rs2910164 genotypes (CG + GG) and risk of psoriasis. Moreover, measuring miR-146a level and knowing patients' genotype could be useful predictors of therapeutic response.

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