Study of Some Enteropathogens Causing Acute Diarrhea in Infants and Children Less than 5 Years Old

Document Type : New and original researches in the field of Microbiology.

Authors

1 Medical Microbiology &Immunology Department, Faculty of Medicine, Tanta University

2 Pediatrics Department, Faculty of Medicine , Tanta University

Abstract

Background: Diarrheal disorders are a major health issue in pediatrics all over the world. Objectives: To isolate enteropathogens causing diarrhea and determine antibiotic sensitivity pattern. Methodology: 120 stool samples underwent the following procedures; macroscopic and microscopic examination, preparation of wet smear, detection of fecal leucocytes ,carbol-fuchsin staining for detection of Campylobacter, Rota virus detection by immunochromatography, culture of samples on Mackongey agar, XLD, API for lactose non fermenting colonies, Identification of EPEC by polyvalent antisera , O157:H7 E.coli detection using Sorbitol Mackoncey and the isolates obtained were tested for antimicrobial sensitivity. Results: The detected enteropathogens were EPEC (46.6%), Rota Virus (20.8%), Salmonella (14.2%), Shigella (1.7%), K.oxytoca (8.3%)and Pseudomonas (4.2%).The antibiotic resistance pattern was ESBL; EPEC (53%), Salmonella (47%). ESBL and Ampc; EPEC (65%), Shigella (5%) and K.oxytoca (25%). Ampc resistance was EPEC while all Pseudomonas isolates were wild. Conclusions: Rehydration therapy, Rota virus vaccination and antibiotic policy are essential for management of diarrheal cases.

Files

Share

How to cite

Statistics