Detection of NDM-1-producing Klebsiella pneumoniae Isolated from Sohag University Hospital, Upper Egypt

Document Type : New and original researches in the field of Microbiology.

Authors

1 Department of Medical Microbiology and Immunology, Faculty of Medicine, Sohag University, Upper Egypt

2 Department of Chest Diseases and Tuberculosis, Faculty of Medicine, Sohag University, Upper Egypt

Abstract

Background: Resistance to carbapenems is a worldwide problem, NDM-1 carbapenemase is rapidly spreading in many parts of the world. Few studies in Egypt reported the enzyme. Objectives: to detect the NDM-1 in carbapenems resistant K. pneumoniae isolated from patients hospitalized in Sohag University hospital. Methodology: The study was conducted during the period between June 2017 to December 2017. K. pneumoniae was isolated from the clinical samples collected from patients with HAI in Intensive Care Units (ICUs), different wards. These samples included blood, urine, sputum/endotracheal aspirates, and pus. Samples were inoculated on primary culture media. The identification and antibiotic susceptibility testing of all suspected K. pneumoniae isolates were performed by the Vitek2 compact system. Combined disk test using EDTA was used for phenotypic testing of Metallo-β-lactamase production. PCR used to detect specific gene for bla NDM-1. Results: 200 samples were collected. Fifty-four (54) K. pneumoniae were isolated and (28)55% were resistant to imipenem and meropenem by Vitek2 compact system. Ten (35.7%) of them were positive for production of Metallo-β-lactamase by combined disk test with EDTA and eight (28.5%) of them were positive blaNDM-1 gene by PCR. Conclusion: This study is the first report of detection of blaNDM-1in Sohag university hospital, Upper Egypt. Appling of infection control measures is a must to prevent the spread of blaNDM-1. Also, strict antibiotic policy is mandatory to decrease antimicrobial resistance in our hospital.

Keywords

Files

Share

How to cite

Statistics