Impact of Biofilm Production in Methicillin Resistant Staphylococcus aureus among Diabetic Foot Patient

Document Type : New and original researches in the field of Microbiology.

Authors

1 Botany and Microbiology Department – Faculty of Science – Cairo University

2 Researcher at Clinical and Chemical Pathology Department, National Research Center

3 Internal Medicine Department, Faculty of Medicine, Fayoum University

Abstract

Background: Diabetic Foot Infection poses many problems in clinical practice. It is usually polymicrobial, and Staphylococcus aureus is the most common pathogen isolated. Objective:To determine the prevalence of methicillin resistant S.aureus(MRSA) and MRSA biofilm production among diabetic patients with chronic leg ulcers. Methodology: This study included 150 patients suffering from infected diabetic footulcers. We used VITEK 2 system to identify isolated bacteria. Colonies of S.aureus were screened for resistance to methicillin on Mueller–Hinton agar supplemented with oxacillin at 4 μg/mL Antibiotic sensitivity test was investigated using Kirby Bauer Disc Diffusion method. Investigation of biofilm formation was performed by tissue culture plate method. Detection of icaA and icaD genes was investigated by PCR. Results: S.aureus was isolated from 70 (46.6%) patients. Among the 70 S. aureus, 34 (22.6%) were (MRSA), Pseudomonas aeruginosa 36(24.0%), Klebseilla pneumoniae 25(16.6%) and E.coli were 19(12.6%). Twenty eight out of 34 tested MRSA (82.35%) were able to form biofilm. Twenty five isolates (73.3%) were strong biofilm former, 3 isolates (8.8%) were moderate biofilm producer and 6 isolates (17.6%) were non biofilm producers. Twenty two were positive for both icaA and icaD genes, On the other hand eight isolates were negative for both genes. Conclusion: A high prevalence of biofilm producing MRSA was detected in S.aureus isolated from patients with Diabetic foot.

Keywords

Files

Share

How to cite

Statistics