Risk Prediction Genetic Study of Non-HLA Gene NOD2 Three Polymorphisms in Acute Graft versus Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation

Ibrahim, Hanan F. Abd El-Aziz; Ghanem, Azaa M. Shawky; Shehata, Maha I.; Fathy, Gamal EL Din M.

doi:10.21608/ejmm.2018.285602

Risk Prediction Genetic Study of Non-HLA Gene NOD2 Three Polymorphisms in Acute Graft versus Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation

Document Type : New and original researches in the field of Microbiology.

Authors

¹ Microbiology and Immunology Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo-Egypt

² Hematology and Bone Marrow Transplantation Department, Naser Institute, Cairo-Egypt

10.21608/ejmm.2018.285602

Abstract

Background: Graft-versus-host disease (GVHD) has been associated with non- human leukocyte antigen (HLA) gene polymorphisms after allogenic Hematopoietic stem cell transplantation (aHSCT). Objective: This study aims to investigate the role of three polymorphisms in Nucleotide-binding Oligomerisation Domain (NOD2) gene on the occurrence / severity of acute GVHD among Egyptian population. Methodology: A total of 64 patients and their corresponding donors who underwent aHSCT from HLA-identical matched siblings were genotyped by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Results were confirmed by automated laser sequencer. Results: There was highly statistical significant association between NOD2 recipient l007fs allele and higher incidence of aGVHD p= 0.006. There was no significant association between NOD2 donor l007fs allele, G908R allele in donors and recipients, R702W in donors and recipients with incidence and severity of aGVHD. Conclusion: This research an original pilot study to demonstrate that the NOD2 SNP13 (Leu1007fs) is a possible risk factor for aGVHD among Egyptian population.

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