Correlation between Interleukin-22 (IL- 22) Genetic Polymorphism and Inflammatory Bowel Disease

Mohamed, Rania A.; Moustafa, Nouran M; Elessawy, Hagar A.; Abdelwahed, Marwa A.; Mahmoud, Fatma M.

doi:10.21608/ejmm.2023.299698

Correlation between Interleukin-22 (IL- 22) Genetic Polymorphism and Inflammatory Bowel Disease

Document Type : New and original researches in the field of Microbiology.

Authors

¹ Medical Microbiology & Immunology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt

² Basic Medical Science Department, Faculty of medicine, Dar Al Uloom University, Riyadh, Saudi Arabia

³ Internal Medicine Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt

⁴ Clinical Pathology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt

10.21608/ejmm.2023.299698

Abstract

Background: Inflammatory bowel disease belongs to digestive system chronic inflammatory disorders of unknown etiology. This disease is represented with two considerable conditions ulcerative colitis and Crohn’s disease. Novel studies illustrated that interleukine-22 has prominent effect on inflammatory processes during the disease. This research aimed to detect the correlation of IL-22 gene polymorphism (rs2227485) with inflammatory bowel disease. Methods: A Case-control study was conducted at internal medicine and gastroenterology outpatient clinics and patients admitted to gastroenterology inpatient ward, Ain Shams university Hospital. Genomic DNA was extracted and amplified using TaqMan Genotyping master mix and ready-made TaqMan SNP Genotyping assay. Results: Patients groups were presented in two groups (30 patients diagnosed as UC and 30 patients with CD) and 30 control subjects. Correlation between genotype & allele frequency among the study groups showed no statistically significant differences between the three groups (P = 0.925). C allele and T allele had been detected in almost all patients and controls with CT genotype predominance. There was a statistically significant differences between both genotypes with Clinical Score (Mayo) in UC, while there was a non-statistically significant differences between both genotypes with Clinical Score (CDAI) in CD. Conclusion: There is no statistically significant correlation between IL-22 gene polymorphism (rs2227485) and the susceptibility to inflammatory bowel disease. More researches are demanded to detect the actual role of IL-22 polymorphism in inflammatory bowel disease.

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