Document Type : New and original researches in the field of Microbiology.
Authors
1
Department of Medical Microbiology and Immunology, Faculty of Medicine, Tanta University, Tanta, Egypt; Department of Basic &Clinical Oral Sciences &, Faculty of Dental Medicine, Umm Al-Qura University, Makkah, Saudi Arabia
2
Department of Medical Microbiology and Immunology, Faculty of Medicine, Tanta University, Tanta, Egypt; Infection Control and Epidemiology Department, King Abdullah Medical City, Makkah, Saudi Arabia,
3
Microbiology Laboratory Supervisor, Security Forces Hospital Makkah, Saudi Arabia
4
Quality Management Supervisor, Security Forces Hospital Makkah, Saudi Arabia
5
Laboratory Quality Coordinator, Security Forces Hospital Makkah, Saudi Arabia
6
Professor and Consultant of Medical Microbiology, Security Forces Hospital Makkah, Saudi Arabia
Abstract
Background: Blood culture contamination is a global problem that heeds all healthcare settings and has many negative impacts. Objectives: to compare the effectiveness of the two implemented interventions on blood culture contamination rates. Methodology: The blood culture reports of specimens received by the microbiology laboratory during the study period were grouped into 3 groups; pre-intervention group, post-intervention I group after educational lectures and practical workshops, post-intervention II group after implementation of the same intervention I together with individual counseling for staff identified as having obtained contaminated specimens. The contamination rates were evaluated and compared to the target and as regards departments and organisms. Results: After intervention I, there was a 31.56% reduction rate while after post-intervention II there was a 56.8% reduction from the pre-intervention rate. The total number of contaminants showed a highly significant difference between pre-intervention and post-intervention I & between post-interventions I and II (p=0.001) and an extremely highly significant difference between pre-intervention and post-intervention II (p=0.0001). The highest rate of contamination was found in the emergency department followed by ICUs. The contaminants were coagulase-negative staphylococci (CoNS) (82.8%, 92.6%, 93.3%) micrococci (9.7%, 5.5% 6.7%), anthracoid (4.9%,1.2%, 0%) and Corynebacterium spp. (2.6%, 0.6%, 0%) in the three groups pre or post interventions respectively. Conclusion: Intervention II proved to be more effective in reducing blood culture contamination rate. So, it is recommended to continuously track the contamination rate and train the staff on the best practice together with disciplinary counseling for those who frequently withdraw contaminated blood culture specimens.
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