Invasive Versus Noninvasive Methods for Diagnosis of Helicobacter Pylori infection

Document Type : New and original researches in the field of Microbiology.

Authors

1 Microbiology and Immunology Department, Faculty of Medicine, Benha University, Benha, Egypt

2 Internal Medicine Department, Faculty of Medicine, Benha University, Benha, Egypt

3 Microbiology and Immunology Department, Faculty of Medicine, Ain Shams University, Egypt

Abstract

Background: Helicobacter pylori (H. pylori) is one of the most widespread infectious organisms in the world that is related to many gastrointestinal diseases. There are numerous techniques available at the present time for identifying infections caused by H. pylori, each has advantages and disadvantages. Objectives: To compare invasive versus noninvasive techniques used for diagnosis of H. pylori infections. Methodology: The study was performed on 100 patients complaining of gastrointestinal problems undergoing GIT endoscopy admitted to Gastroenterology Department, Benha University Hospital. Two samples of gastric biopsy were taken from each patient ; one  used for bacteriological culture and one used for Polymerase chain reaction (PCR), 100 stool samples were taken for stool antigen test (SAT), and 100 serum samples for Enzyme-Linked Immunosorbent Assay ( ELISA). Results: Out of 100 biopsy samples, 71 (71%) had been positive for PCR, 70 (70 %) had been positive for culture. Out of 100 stool sample, 64 (64%) had been positive for SAT. Out of 100 serum samples, 68 (68%) had been positive for ELISA. In comparison with PCR, the culture showed 98.6 % sensitivity, 89.7 % specificity, and total accuracy of 96% then SAT with 88% accuracy and ELISA showed 86% accuracy. Conclusion: Considering PCR as the gold standard method for H. pylori diagnosis, the culture showed the highest overall performance followed by SAT and ELISA.

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