Influence of Diclofenac and Pheniramine on the Virulence of Different Clinical Isolates of Pseudomonas aeruginosa

Document Type : New and original researches in the field of Microbiology.

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Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Egypt

Abstract

Objectives: This study aimed to examine the effect of commonly used non-antibiotic drugs e.g. diclofenac and pheniramine, Background: Treatment of Pseudomonas aeruginosa infections, on antibiotic resistance and the virulence of this pathogen. The antibiotics: gentamicin, cefepime, ciprofloxacin and meropenem were investigated in this study. Methodology: In this work we selected the following final concentrations: 1,4µg/ml for diclofenac and 0,173µg/ml for pheniramine to be used in combination with antibiotics or alone for investigation of their effects on antibiotic resistance and virulence of 20 isolates of P. aeruginosa. Results: The drugs either increased or decreased antibiotic resistance in only 3 isolates of the 20 isolates which indicated that the investigated drugs did not affect the antibiotic resistance when used in combinations. Interestingly, our study demonstrated that both diclofenac and pheniramine increased the haemolytic activity of the investigated isolates. On the other side, no overall final increasing or decreasing effect could be observed regarding the effect of diclofenac or pheniramine on the proteolytic activity of the investigated isolates. The results were confirmed by Real time PCR  diclofenac showed a significant down- regulation of virulance genes namely; algD, plch and toxA apperently  in case of combination  with ciprofloxacin and to a lower extent when combined with gentamicin. Conclusion: Resistance of P. aeruginosa to Gentamicin and Ciprofloxacin may be successfully affected by combining these antibiotics with Diclofenac.

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