Document Type : New and original researches in the field of Microbiology.
Authors
1
Microbiology and Immunology Department, Faculty of Medicine, Benha University, Benha, Egypt
2
Hepatology, Gastroenterology and Infectious Diseases Department, Faculty of Medicine, Benha University, Benha, Egypt
3
Clinical and Chemical Pathology Department, Faculty of Medicine, Benha University, Benha, Egypt; Faculty of Medicine, King Salman International University (KSIU), El Tur Branch, Egypt
4
Clinical and Chemical Pathology Department, Faculty of Medicine, Benha University, Benha, Egypt
5
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Benha University, Benha, Egypt
6
Internal Medicine Department, Faculty of Medicine, Benha University, Benha, Egypt
Abstract
Background: Hepatocellular carcinoma (HCC) is a global health problem. HCC is the fourth common malignancy in Egypt. It is a crucial medical need to predict hepatocellular carcinoma development in cirrhotic liver patients. Objectives: this study aims to investigate the association between TLR4 gene polymorphism (rs2149356) and the probability of HCC development among chronic hepatitis C virus infected patients and clarify the role of serum IL-17 in patients with end stage liver disease and HCC in HCV infected patients. Methodology: the study included 25 patients with chronic hepatitis C infection, 25 patients with chronic hepatitis C infection and HCC, and 25 apparently healthy control subjects as controls. All participants undergone full clinical and laboratory assessment. TLR4 (rs2149356) G/T polymorphism genotyping by PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism) and serum IL-17 detection by ELISA (enzyme-linked immunosorbent assay). Results: TLR4 (rs2149356) GG genotype was significantly associated with chronic HCV with HCC patients (72%) and chronic HCV patients (48%), while GT genotype was associated with healthy controls (48%) (P-value <0.001). Serum IL-17 was significantly elevated in chronic HCV with HCC patients than chronic HCV and control subjects (P-value <0.001). Serum IL-17 was an excellent predictor for HCC development at a cut-off value of 128.1 pg/dL with 96% sensitivity and 82% specificity. Conclusion: TLR4 (rs2149356) polymorphism and IL-17 have an important role in immunopathogenesis of HCC in chronic HCV infected patients.
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