Bisphenol A Effect on T regulatory and T helper 17 Related Cytokines in Female Mice

Thabet, Doaa M.A; Badary, Mohamed S.; Ibrahim, Maggie A.; Nafee, Amany M.A.M.

doi:10.21608/ejmm.2024.333956

Bisphenol A Effect on T regulatory and T helper 17 Related Cytokines in Female Mice

Document Type : New and original researches in the field of Microbiology.

Authors

¹ Department of Microbiology, Faculty of Pharmacy, Assiut University

² Department of Microbiology and Immunology, Faculty of Medicine, Assiut University

10.21608/ejmm.2024.333956

Abstract

Background: In recent years there has been increasing concern about the potential health effects of exposure to Bisphenol A, especially in pregnant women and their offspring. The potential health effects of BPA exposure have been studied extensively in animal models. There is evidence to suggest that exposure to this compound can have a range of adverse health effects, including reproductive and developmental malformations, metabolic disorders, and immune dysfunction. Objectives: To determine the effect of Bisphenol A consumption on regulatory T cells and Th17 cell-associated cytokines in female mice, as well as whether Bisphenol A exposure could cause abortion in pregnant mice. Methodology: The study included 60 female mice divided into four groups; each group contained 15 female mice. The first group is the control, non-pregnant female mice, which take water containing 1% ethanol. The second group, female mice who aren҆t pregnant, which given 5 mg/ml/kg of a BPA solution. The third group is a control group of pregnant female mice who take water containing 1% ethanol. The fourth group, pregnant female mice, were given 5 mg/ml/kg of BPA solution. At the end of the trial, blood samples were taken and serum concentrations of Il-10, TGF-beta, and IL-17 were measured using ELISA kits. Results: In both pregnant and non-pregnant female mice, the experimental group exposed to BPA had considerably lower levels of TGF-beta and -IL-10 than the control group. Simultaneously, the experimental group had significantly higher levels of IL-17 than the control group. There was also a substantial change in TGF-beta, IL-10, and IL-17 levels between non-pregnant and pregnant mice. Conclusion: BPA exposure during pregnancy can result in aberrant T reg. and Th 17 cell-related cytokines and abortion in female mice.

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