Relationship between Biofilm Formation and Production of Extended-Spectrum β-lactamases and Metallo-β-lactamases among Gram-negative Bacilli

Document Type : New and original researches in the field of Microbiology.

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Department of Medical Microbiology & Immunology, Faculty of Medicine, Cairo University

Abstract

Background: Gram-negative bacilli are important pathogens of hospital-acquired infections as they can survive in hospital environment. They show a wide range of antibiotic resistance. Objectives: To detect biofilm, ESBL and MBL production among Gram-negative bacilli, to correlate their relation together and assess their antibiotic resistance pattern. Methodology: Two hundred isolates of Gram-negative bacilli, collected from different clinical samples of hospitalized patients at Cairo University Hospitals, were subcultured on MacConkey’s agar and identified by conventional methods. Antibiotic susceptibility testing was performed using Kirby-Bauer disc diffusion method. The ability of biofilm production was assessed using microtiter plate method. Results:  Gram-negative bacilli isolates were most frequently recovered from urine samples (53%). Biofilm formation was found in 47% of the isolates. Furthermore, strong biofilm-forming category was detected in 2% of the isolates, while moderate and weak biofilm-forming categories were detected in 13% and 85% respectively. Production of ESBL and MBL among Gram-negative bacilli isolates was 39.5 % and 49% respectively. Conclusions: Biofilm formation was associated with MBL production among Gram-negative isolates. Moreover, ESBL and MBL producers were more resistant to antibiotics than non ESBL and non MBL producers.

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