Document Type : New and original researches in the field of Microbiology.
Authors
1
Department of Clinical and Chemical Pathology, Medical Research and Clinical Studies Institute, National Research Centre, Cairo, Egypt
2
Department of Molecular Genetics and Enzymology, Human Genetics and Genome Research Institute, National Research Centre, Cairo, Egypt
3
Rheumatology Department, Kasr Alainy Faculty of Medicine, Cairo University, Cairo, Egypt
4
Internal Medicine Department, Medical Research and Clinical Studies Institute, National Research Centre, Cairo, Egypt
5
Clinical and Chemical Pathology Department, Kasr Alainy Faculty of Medicine, Cairo University, Cairo, Egypt
Abstract
Background: An autoimmune connective tissue illness that affects several organs and lowers a patient's quality of life is the juvenile systemic lupus erythematosus (JSLE) and associated juvenile lupus nephritis (JLN). Interleukin-19 (IL-19) has both anti- and pro- inflammatory actions. LC3-associated phagocytosis (LAP) regulates immune responses to dying cells. Polymorphisms disrupting both mechanisms have been correlated to autoimmune diseases. Objective: to determine whether single-nucleotide polymorphisms (SNPs) of rs2243188 (IL-19) and/or rs933717 (MAP1LC3B) exhibit meaningful relationship with JSLE and /or JLN in the Egyptian population. Methodology: forty-seven JSLE patients and fifty matched healthy controls (HC) were included. SNPs identification was done using allelic discrimination real-time polymerase chain reaction (PCR) technology. Results: Genotyping of rs933717 and rs2243188 showed no statistical difference between the two studied groups with (P=0.206 and 0.468 respectively). Similarly, there was no significant difference in allele frequencies in neither rs933717 nor rs2243188 (P= 0.362 and 0.552 respectively). Twenty-nine out of the 47 JSLE patients (61.7%) displayed JLN symptoms. Similarly, JLN revealed no significant difference in either the genotype distribution or the allele frequency in the dominant/negative model for both genes. Likewise, there was no significant difference in the frequency of any allele concerning rs933717 or rs2243188, (P= 0.309 and 0.196 respectively). Conclusion: Interleukin-19 rs2243188 and MAP1LC3B rs933717 polymorphisms do not seem to play any role in the pathogenesis of JSLE or JLN in Egyptian population.
Keywords
Main Subjects
)
View on SCiNiTO