Evaluation of the Antagonistic Activity of Lactobacillus strains against Pathogenic Organisms isolated from Various Infected Wounds: An In Vitro Study

Document Type : New and original researches in the field of Microbiology.

Authors

1 Department of Microbiology and Immunology, Faculty of Medicine, Tanta University, Egypt

2 Department of Plastic and Reconstructive Surgery, Faculty of Medicine, Tanta University

Abstract

Background: With the serious global threat of antimicrobial resistance, finding therapeutic alternatives has become a critical priority. Probiotics appear to be promising treatments due to their known natural inhibitory effects on various pathogens. Objectives: To evaluate the potential antagonistic effects of the probiotic Lactobacillus plantarum and Lactobacillus rhamnosus as well as their cell-free supernatant (CFS) and the synergistic interaction with commercial prebiotics like Fructooligosaccharides (FOS) on different pathogens isolated from infected wounds. Methodology: Identification of the pathogenic microorganisms was done. The tissue culture plate method evaluated the biofilm formation ability among the isolated pathogens.  The antimicrobial activity of L. plantarum and L. rhamnosus and their CFS were investigated by radial streak technique and agar well diffusion. The anti-biofilm effect of CFS of lactobacilli was evaluated using tissue culture plate assay. Results: Seventy-two isolates were identified, 73.6% were Gram-negative, and the most prevalent was Klebsiella pneumoniae. Biofilm formation was identified in 69.5 % of the isolates, among them E. coli, Acinetobacter baumannii, and Proteus mirabilis were the most frequent biofilm-forming organisms. L. plantarum, L. rhamnosus and their CFS exhibited antimicrobial and antibiofilm activity against all tested pathogens with variable degrees. The addition of FOS enhanced both lactobacilli's antimicrobial and antibiofilm properties. Conclusion: The probiotics used showed significant antagonistic activity against various pathogens paving the way to be used as a new treatment option.

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