MicroRNA 29 gene Expression and Progesterone Receptor Values in Iraqi Women with Breast Cancer

Document Type : New and original researches in the field of Microbiology.

Authors

Faculty of Science, Biology Department, Kufa University, Iraq

10.21608/ejmm.2024.319913.1337

Abstract

Background: Globally as well as in Iraq, Breast cancer (BC) is the most common malignant tumor among malignancies that affect women and one of the most deadly illnesses that affect women. MicroRNAs (miRNAs) are 18–25-nucleotides regulatory non coding short RNAs that post-transcriptionally control the expression of genes to perform a wide range of essential functions in various biological processes. Objectives: The study aimed to evaluate the miR-29a expression level and association with breast cancer as well as to find out the relationship between miR29 and Progesterone receptor. Methodology: There were 74 of blood samples (54 breast cancer patients and 20 healthy control group).RNA extracted from patients and controls to synthesize complementary DNA(cDNA).Gene expression of miR29a was carried out using the quantitative reverse transcription polymerase chain reaction (RT-qPCR). Results: The results revealed that miR-29a gene expression in patients with breast cancer was (1.276851852), while in healthy people, the 2^-(∆∆Ct) was equal to zero, with a standard error of (0.281) and a dispersion deviation of (2.069) in comparison with control group. It was noted that women with breast cancer had an increased concentration of progesterone receptors, where the average concentration for healthy people was 0.348. Conclusion: From these findings we concluded that miR29 can serve as biomarker, diagnostic or predictive tool upon diagnosing breast cancer in the medical laboratories and also for treatment development. There was a significant association between mir29a and PR levels in this study.

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