Activity of Nigella Sativa Oil on Multidrug Resistant Organisms Isolated from Diabetic Foot Patients

Document Type : New and original researches in the field of Microbiology.

Authors

1 Medical Microbiology and Immunology Department, Faculty of Medicine, Tanta University, Tanta, Egypt

2 Vascular and Endovascular Surgery Department, Faculty of Medicine, Tanta University, Tanta, Egypt

Abstract

Background: Multidrug-resistant (MDR) organisms are becoming more prevalent worldwide, which is an issue in poor nations that depend on natural resources, especially traditional and herbal plant sources. Nigella sativa oil (NSO) is a strong antibacterial that could be an excellent option. Objective: The aim of the present work is to determine the in vitro antibacterial efficacy of NSO against MDR microbes isolated from diabetic foot ulcers. Methodology: By using the agar well diffusion method to evaluate the zone of inhibition of NSO, the antibacterial impact of NSO against MDR bacteria of diabetic foot ulcers was ascertained. The tube dilution method was used to find the minimal inhibitory concentration (MIC), and sub-culturing tubes that showed no discernible growth or turbidity in the MIC allowed for the determination of the minimal bactericidal concentration (MBC).  Results:  NSO produced zone of inhibition ranged from 0 mm. to 28 mm., MIC ranged from 3% to 100% and MBC ranged from 6% to 100% against different MDR bacteria of diabetic foot ulcer. Bacterial outcomes and inhibition zones made by NSO against different isolated organisms were insignificantly different between Gram-positive and Gram-negative organisms. Conclusion: NSO had antibacterial effects against MDR Gram-positive and Gram-negative organisms. It showed significant synergism in combination with different antibiotics. 

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