Intratonsillar Molecular Detection of some Herpesviruses with a Histopathological Study

Document Type : New and original researches in the field of Microbiology.

Authors

1 Basrah Directorate of Education, Ministry of Education, Iraq

2 College of Science, University of Basrah, Basrah, Iraq

Abstract

Background: Recurrent tonsillitis (CT) is a parenchymal infection of the palatine tonsils that is identified based on clinical criteria, some studies show a close relation between viral infections and tonsillitis. Objectives: The aim of our study is to determine the prevalence of some human herpesviruses (HHV) including HHV-4, HHV-6 and HHV-7 using the multiplex  polymerase chain reaction (mPCR) technique and to characterize the association between viruses and recurrent tonsillitis to evaluate the histopathological changes in tonsillar tissues among patients with tonsillitis Methodology: A total of (100) samples from patients of Basrah general hospital during period (Sep. 2023 to Feb. 2024) from both sexes were collected after surgical interventions from (54) males and (46) females with age ranged (2-50) years with regard to clinical information and molecular detection and histopathological study. Results: Our results showed that EBV (HHV-4 DNA) was detected in 20% samples, while positive results of HHV-6 DNA was 16%, tonsillitis  in  children  infected  with  HHV  type  6  is  characterized  by  a  longer course  of the disease, which leads to a longer hospital treatment and  the age group (6-10) years was more infected than other age groups, and the tonsillitis was predominantly in males more than women, while HHV-7 DNA was not amplified. Conclusion:  The current study concludes that the most common virus in the tonsils was EBV, then HHV6, with HHV7 not being diagnosed.

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