Granzyme B Gene Polymorphism (rs11539752) and Breast Cancer in Egyptian Women

Document Type : New and original researches in the field of Microbiology.

Authors

1 Zoology Department, Faculty of Science, Menoufia University, Shebin El-Kom, Menoufia, Egypt

2 General Surgery, Faculty of Medicine, Benha University, Egypt

3 Clinical Pathology, National Cancer Institute, Cairo University, Egypt

4 Natural Resources Department, Faculty of African Postgraduate Studies, Cairo University, Egypt

Abstract

Background: Breast cancer (BC) is the most common type of female cancer in Egypt. Granzyme B (GrB) is primarily found in cytotoxic granules and has traditionally been considered the most abundant granzyme. However, recent research has revealed various other crucial roles for GrB. Specifically, GrB expression in both normal epithelial cells and cancer cells affects extracellular matrix remodeling, epithelial-to-mesenchymal transition, and fibrosis. Objectives: In this study, we investigated whether a common genetic variation in the gene encoding GrB, consisting of one single nucleotide polymorphisms (rs11539752), is associated with breast cancer risk in Egyptian women. Methodology: This study included 195 participants, with 103 diagnosed with breast cancer and 92 serving as controls.  Genotyping of the Granzyme B gene variants (rs11539752) was conducted using tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). Results: We find that CC genotype was higher in breast cancer group than control group and GG genotype was higher in breast cancer group than control group (p>0.05) and odds ratio (95% confidence interval) in GG genotype between breast cancer and control groups 0.971 (0.939-1.004). Conclusion: The variant allele (rs11539752) couldn’t be considered as a predictive factor for breast cancer development in Egyptian women.

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